Delivery of Vaccines by Biodegradable Polymeric Microcapsules with Bioadhesion Properties
Author | : Debra J. Trantolo |
Publisher | : |
Total Pages | : 37 |
Release | : 1997 |
ISBN-10 | : OCLC:227990880 |
ISBN-13 | : |
Rating | : 4/5 (80 Downloads) |
Book excerpt: Vaccine research has traditionally focused on the induction of systemic immunity through parenteral immunization. However, parenteral vaccines do not induce mucosal immunity. The presence of a common mucosal immune system which may be stimulated by oral immunization has been established. Oral immunization has been shown to result in the induction of sigA and T cell responses a mucosal sites. Thus, oral immunization may be used for the induction of protective immunity against not only pathogens of the GI tract, but also of pathogens that infect at other mucosal sites. One of the most crucial factors in the success of oral immunization is the selection of the antigen delivery system. The objective of this Phase II SBIR proposal is to develop a PLGA-based delivery system for oral vaccines: one of military interest and one of private sector interest. The first year of this project has focused in two areas: (1) development of the necessary technology to obtain the correct particle size of the polymer matrices; and (2) in vitro bioadhesion studies. A combination of impeller milling, air milling, and microfluidizer technology has returned particles of less than 10 microns which will help accelerate the uptake of antigens. The use of 10% gelatin in the formulations has increased bioadhesion considerably.